{"id":65166,"date":"2023-02-26T10:50:08","date_gmt":"2023-02-26T03:50:08","guid":{"rendered":"https:\/\/asprou.com\/?p=65166"},"modified":"2023-04-06T15:46:34","modified_gmt":"2023-04-06T08:46:34","slug":"ky-thuat-sac-ky-trong-phan-tich-dinh-luong-va-ung-dung","status":"publish","type":"post","link":"https:\/\/asprou.com\/uncategorized-vi\/ky-thuat-sac-ky-trong-phan-tich-dinh-luong-va-ung-dung\/","title":{"rendered":"K\u1ef8 THU\u1eacT S\u1eaeC K\u00dd TRONG PH\u00c2N T\u00cdCH \u0110\u1ecaNH L\u01af\u1ee2NG V\u00c0 \u1ee8NG D\u1ee4NG"},"content":{"rendered":"\n
  1. K\u1ef9 thu\u1eadt s\u1eafc k\u00fd<\/strong><\/li><\/ol>\n\n\n\n

    K\u1ef9 thu\u1eadt s\u1eafc k\u00fd l\u00e0 m\u1ed9t quy tr\u00ecnh cho ph\u00e9p t\u00e1ch ri\u00eang c\u00e1c ch\u1ea5t tan trong h\u1ee3p ch\u1ea5t nh\u1edd vi\u1ec7c cho pha \u0111\u1ed9ng chuy\u1ec3n \u0111\u1ed9ng li\u00ean t\u1ee5c v\u00e0 trong pha n\u00e0y c\u00e1c ch\u1ea5t ri\u00eang bi\u1ec7t th\u1ec3 hi\u1ec7n m\u1ee9c \u0111\u1ed9 linh \u0111\u1ed9ng kh\u00e1c nhau do c\u00f3 s\u1ef1 kh\u00e1c bi\u1ec7t v\u1ec1 m\u1ee9c \u0111\u1ed9 ph\u00e2n b\u1ed1, h\u1ea5p ph\u1ee5, \u0111i\u1ec7n t\u00edch, \u2026 C\u00e1c ch\u1ea5t ri\u00eang bi\u1ec7t sau khi t\u00e1ch ra c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh b\u1eb1ng c\u00e1c ph\u01b0\u01a1ng ph\u00e1p ph\u00e2n t\u00edch kh\u00e1c. \u01afu \u0111i\u1ec3m c\u1ee7a ph\u01b0\u01a1ng ph\u00e1p s\u1eafc k\u00fd l\u00e0 \u0111\u01a1n gi\u1ea3n v\u00e0 t\u00e1ch \u0111\u01b0\u1ee3c c\u00e1c ch\u1ea5t trong h\u1ed7n h\u1ee3p n\u00ean \u0111\u01b0\u1ee3c \u1ee9ng d\u1ee5ng trong c\u00f4ng t\u00e1c nghi\u00ean c\u1ee9u, ph\u00e2n t\u00edch v\u00e0 ki\u1ec3m nghi\u1ec7m thu\u1ed1c.<\/p>\n\n\n\n

    2. S\u1eafc k\u00fd c\u1ed9t<\/strong><\/p>\n\n\n\n

    S\u1eafc k\u00fd c\u1ed9t c\u1ed5 \u0111i\u1ec3n \u0111\u01b0\u1ee3c d\u00f9ng \u0111\u1ec3 t\u00e1ch \u0111\u01a1n ch\u1ea5t ra kh\u1ecfi h\u1ed7n h\u1ee3p. Ng\u00e0y nay \u0111\u00e3 c\u00f3 c\u00e1c ph\u01b0\u01a1ng ph\u00e1p s\u1eafc k\u00fd c\u1ed9t c\u1ea3i ti\u1ebfn nh\u01b0 s\u1eafc k\u00fd tr\u00ean c\u1ed9t b\u1eb1ng ch\u1ea5t d\u1ebbo, s\u1eafc k\u00fd c\u1ed9t nhanh t\u1ea1o \u00e1p su\u1ea5t t\u1eeb tr\u00ean xu\u1ed1ng, s\u1eafc k\u00fd c\u1ed9t ch\u00e2n kh\u00f4ng t\u1ea1o \u00e1p su\u1ea5t ph\u00eda d\u01b0\u1edbi, s\u1eafc k\u00fd c\u1ed9t r\u00e2y ph\u00e2n t\u1eed v\u00e0 s\u1eafc k\u00fd c\u1ed9t ph\u00e2n b\u1ed1. C\u00e1c ph\u01b0\u01a1ng ph\u00e1p c\u1ea3i ti\u1ebfn gi\u00fap cho qu\u00e1 tr\u00ecnh khai tri\u1ec3n di\u1ec5n ra nhanh h\u01a1n, vi\u1ec7c l\u1ea5y m\u1eabu thu\u1eadn ti\u1ec7n, n\u1ea1p \u0111\u01b0\u1ee3c nhi\u1ec1u m\u1eabu h\u01a1n, lo\u1ea1i \u0111\u01b0\u1ee3c mu\u1ed1i ho\u1eb7c \u0111\u01b0\u1eddng ra kh\u1ecfi h\u1ed7n h\u1ee3p, tinh ch\u1ebf s\u1ea3n ph\u1ea9m, ph\u00e2n l\u1eadp c\u1ea5u tr\u00fac d\u1ef1a v\u00e0o k\u00edch th\u01b0\u1edbc. Nh\u00ecn chung trong vi\u1ec7c ki\u1ec3m nghi\u1ec7m thu\u1ed1c, ph\u01b0\u01a1ng ph\u00e1p n\u00e0y \u0111a ph\u1ea7n s\u1eed d\u1ee5ng s\u1eafc k\u00fd c\u1ed9t c\u1ed5 \u0111i\u1ec3n \u0111\u1ed3ng th\u1eddi gh\u00e9p v\u1edbi c\u00e1c ph\u01b0\u01a1ng ph\u00e1p kh\u00e1c \u0111\u1ec3 ph\u00e1t hi\u1ec7n c\u1ea5u tr\u00fac c\u1ee7a ch\u1ea5t sau khi \u0111\u01b0\u1ee3c t\u00e1ch.<\/p>\n\n\n\n

    \u0110i\u1ec1u ch\u1ebf polyme in d\u1ea5u ph\u00e2n t\u1eed l\u00f5i-v\u1ecf cho lincomycin A v\u00e0 \u1ee9ng d\u1ee5ng c\u1ee7a n\u00f3 trong c\u1ed9t s\u1eafc k\u00fd <\/em><\/strong>2<\/sup>.<\/p>\n\n\n\n

    Lincomycin l\u00e0 m\u1ed9t lo\u1ea1i kh\u00e1ng sinh c\u00f3 hi\u1ec7u qu\u1ea3 ch\u1ed1ng l\u1ea1i c\u00e1c sinh v\u1eadt Gram d\u01b0\u01a1ng. Trong qu\u00e1 tr\u00ecnh s\u1ea3n xu\u1ea5t lincomycin, s\u1ea3n ph\u1ea9m thu \u0111\u01b0\u1ee3c c\u00f3 lincomycin A, B, C, D. Lincomycin A l\u00e0 s\u1ea3n ph\u1ea9m ch\u00ednh, c\u00e1c s\u1ea3n ph\u1ea9m c\u00f2n l\u1ea1i l\u00e0 t\u1ea1p ch\u1ea5t. Lincomycin C r\u1ea5t \u00edt v\u00e0 c\u00f3 c\u00f9ng t\u00e1c d\u1ee5ng d\u01b0\u1ee3c l\u00fd v\u1edbi lincomycin A. Lincomycin B l\u00e0 th\u00e0nh ph\u1ea7n t\u1ea1p ch\u1ea5t ch\u00ednh c\u00f3 ho\u1ea1t t\u00ednh th\u1ea5p h\u01a1n v\u00e0 g\u00e2y \u0111\u1ed9c cao h\u01a1n lincomycin A. V\u00ec v\u1eady, h\u00e0m l\u01b0\u1ee3ng c\u1ee7a n\u00f3 trong s\u1ea3n ph\u1ea9m ph\u1ea3i n\u1eb1m trong gi\u1edbi h\u1ea1n quy \u0111\u1ecbnh. \u0110\u1ec3 c\u1ea3i thi\u1ec7n quy tr\u00ecnh s\u1ea3n xu\u1ea5t c\u1ea7n ph\u1ea3i gi\u1ea3m h\u00e0m l\u01b0\u1ee3ng lincomycin B trong nguy\u00ean li\u1ec7u . Vi\u1ec7c lo\u1ea1i b\u1ecf c\u00e1c ch\u1ea5t t\u01b0\u01a1ng t\u1ef1 B, C, D g\u1eb7p nhi\u1ec1u kh\u00f3 kh\u0103n do chi ph\u00ed cao, hi\u1ec7u su\u1ea5t th\u1ea5p v\u00e0 ph\u1ea3i s\u1eed d\u1ee5ng \u0111\u1ebfn c\u00e1c dung m\u00f4i h\u1eefu c\u01a1. Do \u0111\u00f3 ti\u1ebfn h\u00e0nh nh\u1ed1t lincomycin A v\u00e0o polyme nh\u1eb1m d\u1ec5 d\u00e0ng t\u00e1ch ri\u00eang ra kh\u1ecfi c\u00e1c ch\u1ea5t t\u01b0\u01a1ng t\u1ef1. S\u1eafc k\u00fd c\u1ed9t \u0111\u01b0\u1ee3c d\u00f9ng \u0111\u1ec3 \u0111\u00e1nh gi\u00e1 kh\u1ea3 n\u0103ng nh\u1ed1t lincomycin A d\u1ef1a v\u00e0o s\u1ef1 thay \u0111\u1ed5i t\u1ed1c \u0111\u1ed9 d\u00f2ng ch\u1ea3y. \u0110\u1ed3ng th\u1eddi s\u1eed d\u1ee5ng s\u1eafc k\u00fd c\u1ed9t \u0111\u1ec3 t\u00e1ch ri\u00eang ph\u1ee9c h\u1ee3p polymer \u0111\u00e3 nh\u1ed1t lincomycin A ra kh\u1ecfi h\u1ed7n h\u1ee3p.<\/p>\n\n\n\n

    3. S\u1eafc k\u00fd l\u1edbp m\u1ecfng (TLC)<\/strong><\/p>\n\n\n\n

    S\u1eafc k\u00fd l\u1edbp m\u1ecfng l\u00e0 m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p \u0111\u01a1n gi\u1ea3n, \u00e1p d\u1ee5ng \u0111\u1ec3 th\u1eed \u0111\u1ed9 tinh khi\u1ebft, gi\u1edbi h\u1ea1n t\u1ea1p ch\u1ea5t, ph\u00e1t hi\u1ec7n t\u1ea1p, \u0111\u1ecbnh t\u00ednh, b\u00e1n \u0111\u1ecbnh l\u01b0\u1ee3ng v\u00e0 \u0111\u1ecbnh l\u01b0\u1ee3ng. \u1ee8ng d\u1ee5ng r\u1ed9ng r\u00e3i, c\u00f3 th\u1ec3 d\u00f9ng \u0111\u1ec3 ki\u1ec3m tra hi\u1ec7u qu\u1ea3 c\u1ee7a qu\u00e1 tr\u00ecnh chi\u1ebft, t\u00e1ch, tinh ch\u1ebf. S\u1eafc k\u00fd l\u1edbp m\u1ecfng c\u00f3 th\u1ec3 d\u00f9ng \u0111\u1ec3 t\u00ecm c\u00e1c \u0111i\u1ec1u ki\u1ec7n t\u1ed1i \u01b0u cho s\u1eafc k\u00fd l\u1ecfng tr\u00ean c\u1ed9t do thi\u1ebft b\u1ecb \u0111\u01a1n gi\u1ea3n, l\u01b0\u1ee3ng dung m\u00f4i s\u1eed d\u1ee5ng \u00edt, th\u1eddi gian khai tri\u1ec3n ng\u1eafn v\u00e0 s\u1eed d\u1ee5ng l\u01b0\u1ee3ng m\u1eabu r\u1ea5t \u00edt. Nh\u01b0\u1ee3c \u0111i\u1ec3m c\u1ee7a ph\u01b0\u01a1ng ph\u00e1p l\u00e0 \u0111\u1ed9 l\u1eb7p l\u1ea1i th\u1ea5p do h\u1ec7 s\u1ed1 Rf  <\/sub>(h\u1ec7 s\u1ed1 di chuy\u1ec3n) b\u1ecb \u1ea3nh h\u01b0\u1edfng b\u1edfi nhi\u1ec1u y\u1ebfu t\u1ed1 trong qu\u00e1 tr\u00ecnh s\u1eafc k\u00fd, pic b\u1ecb d\u00e3n r\u00f4ng ra do t\u1ed1c \u0111\u1ed9 d\u00f2ng dung m\u00f4i pha \u0111\u1ed9ng th\u1ea5p. \u0110\u1ec3 kh\u1eafc ph\u1ee5c v\u1ea5n \u0111\u1ec1 tr\u00ean nh\u1edd k\u1ef9 thu\u1eadt s\u1eafc k\u00fd cao \u00e1p tr\u00ean m\u1eb7t ph\u1eb3ng \u2013 OPLC v\u00e0 s\u1eafc k\u00fd l\u1edbp m\u1ecfng hi\u1ec7u n\u0103ng cao.<\/p>\n\n\n\n

    OPLC l\u00e0 ph\u01b0\u01a1ng ph\u00e1p s\u1eed d\u1ee5ng \u00e1p su\u1ea5t \u0111\u1ec3 \u0111\u1ecbnh h\u01b0\u1edbng d\u00f2ng ch\u1ea3y pha \u0111\u1ed9ng d\u1eabn \u0111\u1ebfn ti\u1ebft ki\u1ec7m th\u1eddi gian s\u1eafc k\u00fd, ch\u1ea5t ph\u00e2n t\u00edch v\u1eabn \u0111\u01b0\u1ee3c \u0111\u01b0a l\u00ean b\u1ea3n m\u1ecfng nh\u01b0 TLC. OPLC th\u01b0\u1eddng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng ch\u1ee7 y\u1ebfu trong s\u1eafc k\u00fd ch\u1ebf h\u00f3a. Khi so s\u00e1nh gi\u1eefa ph\u01b0\u01a1ng ph\u00e1p TLC v\u00e0 OPLC \u0111\u1ec3 ph\u00e1t hi\u1ec7n m\u1ed9t s\u1ed1 th\u00e0nh ph\u1ea7n \u0111\u1eb7c tr\u01b0ng c\u1ee7a c\u00e1c lo\u1ea1i tinh d\u1ea7u, ph\u01b0\u01a1ng ph\u00e1p OPLC cho th\u1ea5y kh\u1ea3 n\u0103ng ph\u00e1t hi\u1ec7n t\u1ed1t h\u01a1n \u0111\u1ed3ng th\u1eddi t\u1ed1n \u00edt dung m\u00f4i v\u00e0 c\u00f3 \u0111\u1ed9 l\u1eb7p l\u1ea1i cao h\u01a1n 4<\/sup>.<\/p>\n\n\n\n

    V\u00ed d\u1ee5 v\u1ec1 kh\u1ea3 n\u0103ng ph\u00e1t hi\u1ec7n t\u1ea1p ch\u1ea5t ph\u00e2n h\u1ee7y trong vi\u00ean n\u00e9n methylprednisolon<\/strong> 3<\/sup>.<\/p>\n\n\n\n

    B\u00e0n m\u1ecfng: Silica gel GF254<\/sub>.<\/p>\n\n\n\n

    Dung m\u00f4i khai tri\u1ec3n: Dicloromethan – ether – methamol- n\u01b0\u1edbc (77 : 15 : 8 : l ,2).<\/p>\n\n\n\n

    Dung m\u00f4i h\u00f2a m\u1eabu: Cloroform – methanol (9 : 1).<\/p>\n\n\n\n

    Dung d\u1ecbch th\u1eed: L\u1eafc m\u1ed9t l\u01b0\u1ee3ng b\u1ed9t vi\u00ean t\u01b0\u01a1ng \u1ee9ng v\u1edbi 20 mg methylpredniso\u1ec9on v\u1edbi 2 ml dung m\u00f4i h\u00f2a m\u1eabu trong 15 ph\u00fat. Ly t\u00e2m v\u00e0 l\u1ea5y d\u1ecbch trong.<\/p>\n\n\n\n

    Dung d\u1ecbch \u0111\u1ed1i chi\u1ec3u (\u1ec9): Pha lo\u00e3ng m\u1ed9t th\u1ec3 t\u00edch dung d\u1ecbch th\u1eed th\u00e0nh 50 th\u1ec3 t\u00edch b\u1eb1ng dung m\u00f4i h\u00f2a m\u1eabu.<\/p>\n\n\n\n

    Dung d\u1ecbch d\u1ed1i chi\u1ebfu (2): Pha lo\u00e3ng 1 th\u1ec3 t\u00edch dung d\u1ecbch \u0111\u1ed1i chi\u1ebfu (1) th\u00e0nh 4 th\u1ec3 t\u00edch b\u1eb1ng dung m\u00f4i h\u00f2a m\u1eabu.<\/p>\n\n\n\n

    Dung d\u1ecbch \u0111\u1ed1i chi\u1ebfu (3): H\u00f2a tan 1 mg hydrocortison trong h\u1ed7n h\u1ecdp g\u1ed3m 0,1 ml dung d\u1ecbch th\u1eed v\u00e0 0,9 ml methanol .<\/p>\n\n\n\n

    C\u00e1ch ti\u1ebfn h\u00e0nh: Ch\u1ea5m ri\u00eang bi\u1ec7t l\u00ean b\u1ea3n m\u1ecfng 5 \u03bcl m\u1ed7i dung d\u1ecbch tr\u00ean. Tri\u1ec3n khai s\u1eafc k\u00fd \u0111\u1ebfn khi dung m\u00f4i \u0111i \u0111\u01b0\u1ee3c 15 cm. L\u1ea5y b\u1ea3n m\u1ecfng ra v\u00e0 \u0111\u1ec3 kh\u00f4 ngo\u00e0i kh\u00f4ng kh\u00ed. Quan s\u00e1t d\u01b0\u1edbi \u00e1nh s\u1ea3ng t\u1eed ngo\u1ea1i \u1edf b\u01b0\u1edbc s\u00f3ng 254 nm. B\u1ea5t k\u1ef3 v\u1ebft ph\u1ee5 n\u00e0o tr\u00ean s\u1eafc k\u00fd \u0111\u1ed3 c\u1ee7a dung d\u1ecbch th\u1eed c\u0169ng kh\u00f4ng \u0111\u01b0\u1ee3c \u0111\u1eadm h\u01a1n v\u1ebft thu \u0111\u01b0\u1ee3c tr\u00ean s\u1eafc k\u00fd \u0111\u1ed3 c\u00f9a dung d\u1ecbch \u0111\u1ed1i chi\u1ebfu (1) (2 %) v\u1ea3 kh\u00f4ng c\u00f3 qu\u00e1 m\u1ed9t v\u1ebft nh\u01b0 v\u1eady \u0111\u1eadm h\u01a1n v\u1ebft thu \u0111\u01b0\u1ee3c tr\u00ean s\u1eafc k\u00fd \u0111\u1ed3 c\u1ee7a dung d\u1ecbch \u0111\u1ed1i chi\u1ebfu (2) (0,5 %). Ph\u00e9p th\u1eed ch\u1ec9 c\u00f3 gi\u00e1 tr\u1ecb khi tr\u00ean s\u1eb3c k\u00fd \u0111\u1ed3 c\u1ee7a dung d\u1ecbch \u0111\u1ed5i chi\u1ec3u (3) c\u00f3 2 v\u1ebft ch\u00ednh g\u1ea7n nhau nh\u01b0ng t\u00e1ch r\u1eddi nhau.<\/p>\n\n\n\n

    4. S\u1eafc k\u00fd l\u1edbp m\u1ecfng hi\u1ec7u n\u0103ng cao (HPTLC)<\/strong><\/p>\n\n\n\n

    HPTLC l\u00e0 ph\u01b0\u01a1ng ph\u00e1p c\u1ea3i ti\u1ebfn c\u1ee7a TLC v\u00e0 OPLC nh\u1eb1m kh\u1eafc ph\u1ee5c c\u00e1c nh\u01b0\u1ee3c \u0111i\u1ec3m c\u1ee7a c\u00e1c ph\u01b0\u01a1ng ph\u00e1p tr\u01b0\u1edbc. Nh\u01b0ng ph\u01b0\u01a1ng ph\u00e1p OPLC v\u1eabn ph\u1ed5 bi\u1ebfn h\u01a1n HPTLC do ti\u1ebft ki\u1ec7m \u0111\u01b0\u1ee3c th\u1eddi gian ph\u00e2n t\u00edch, ch\u1ecd \u0111\u01b0\u1ee3c t\u1ed1c \u0111\u1ed9 pha \u0111\u1ed9ng \u0111\u1ec3 t\u1ed1i \u01b0u vi\u1ec7c t\u00e1ch.<\/p>\n\n\n\n

    T\u1ed1i \u01b0u h\u00f3a v\u00e0 th\u1ea9m \u0111\u1ecbnh quy tr\u00ecnh \u0111\u1ec3 x\u00e1c \u0111\u1ecbnh doxycycline hyclate trong c\u00f4ng th\u1ee9c vi\u00ean nang v\u00e0 vi\u00ean n\u00e9n b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p s\u1eafc k\u00fd l\u1edbp m\u1ecfng hi\u1ec7u n\u0103ng cao (HPTLC) 5<\/sup>.<\/strong><\/p>\n\n\n\n

    Chu\u1ea9n b\u1ecb dung d\u1ecbch chu\u1ea9n<\/em>: Dung d\u1ecbch ti\u00eau chu\u1ea9n c\u1ee7a doxycycline \u0111\u01b0\u1ee3c chu\u1ea9n b\u1ecb b\u1eb1ng c\u00e1ch h\u00f2a tan m\u1ed9t l\u01b0\u1ee3ng ch\u1ea5t chu\u1ea9n t\u01b0\u01a1ng \u0111\u01b0\u01a1ng v\u1edbi 15 mg doxycycline hyclate trong 50 mL methanol v\u00e0 sau \u0111\u00f3 b\u1ed5 sung h\u1ed7n h\u1ee3p acetonitril-metanol (30:70 th\u1ec3 t\u00edch\/th\u1ec3 t\u00edch) t\u1edbi v\u1ea1ch 100 mL.<\/p>\n\n\n\n

    Chu\u1ea9n b\u1ecb dung d\u1ecbch m\u1eabu: <\/em>C\u00e2n 20 vi\u00ean ch\u1ebf ph\u1ea9m vi\u00ean nang ho\u1eb7c vi\u00ean n\u00e9n doxycycline hyclate t\u00ednh kh\u1ed1i l\u01b0\u1ee3ng trung b\u00ecnh v\u00e0 nghi\u1ec1n tr\u1ed9n \u0111\u1ec1u. C\u00e2n m\u1ed9t l\u01b0\u1ee3ng t\u01b0\u01a1ng \u0111\u01b0\u01a1ng v\u1edbi 15 mg doxycycline \u0111\u01b0\u1ee3c chuy\u1ec3n v\u00e0o b\u00ecnh \u0111\u1ecbnh m\u1ee9c 100 mL v\u00e0 th\u00eam 50 ml methanol l\u1eafc \u0111\u1ec1u v\u00e0 sau \u0111\u00f3 \u0111\u1eb7t trong b\u1ec3 si\u00eau \u00e2m trong 10 ph\u00fat. D\u1ecbch chi\u1ebft \u0111\u00e3 l\u1ecdc \u0111\u01b0\u1ee3c chuy\u1ec3n v\u00e0o b\u00ecnh \u0111\u1ecbnh m\u1ee9c 100 mL v\u00e0 pha lo\u00e3ng \u0111\u1ebfn v\u1ea1ch b\u1eb1ng acetonitril-metanol (30:70 70 th\u1ec3 t\u00edch\/th\u1ec3 t\u00edch).<\/p>\n\n\n\n

    \u0110i\u1ec1u ki\u1ec7n thi\u1ebft b\u1ecb v\u00e0 s\u1eafc k\u00fd: <\/em>Thi\u1ebft b\u1ecb Camag HPTLC bao g\u1ed3m d\u1ee5ng c\u1ee5 l\u1ea5y m\u1eabu Linomat V (Camag, Muttenz, Th\u1ee5y S\u0129), \u1ed1ng ti\u00eam 100 \u03bcL (Hamilton-Bonaduz Schweiz, Camag, Th\u1ee5y S\u0129), TLC Scanner III (Camag, Muttenz, Swtzerland), ph\u1ea7n m\u1ec1m winCATS phi\u00ean b\u1ea3n 1.4.0 (Camag , Muttenz, Th\u1ee5y S\u0129) \u0111\u00e3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng trong nghi\u00ean c\u1ee9u. S\u1eafc k\u00fd \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n tr\u00ean Merck silica gel 60 F 254c\u00e1c t\u1ea5m TLC tr\u00e1ng tr\u01b0\u1edbc (20 cm \u00d7 20 cm v\u1edbi \u0111\u1ed9 d\u00e0y 200 \u03bcm; s\u1ed1 l\u00f4: HX389048 v\u00e0 HX398477), gi\u1ea5y b\u00e3o h\u00f2a (Camag, Muttenz, Th\u1ee5y S\u0129) \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng cho c\u00e1c bu\u1ed3ng ph\u00e1t tri\u1ec3n b\u00e3o h\u00f2a. C\u00e1c m\u1eabu \u0111\u01b0\u1ee3c \u00e1p d\u1ee5ng th\u00e0nh c\u00e1c d\u1ea3i d\u01b0\u1edbi d\u00f2ng nit\u01a1 b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng \u1ed1ng ti\u00eam \u03bcL. Qu\u00e1 tr\u00ecnh ph\u00e1t tri\u1ec3n t\u0103ng d\u1ea7n \u0111\u1ebfn kho\u1ea3ng c\u00e1ch 7 cm \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n trong bu\u1ed3ng ph\u00e1t tri\u1ec3n TLC 20 \u00d7 20 cm 2 m\u00e1ng \u0111\u00f4i b\u00e3o h\u00f2a tr\u01b0\u1edbc 30 ph\u00fat (Camag). C\u00e1c b\u1ea3n m\u1ecfng \u0111\u00e3 ch\u1ea5m m\u1eabu \u0111\u01b0\u1ee3c l\u00e0m kh\u00f4 b\u1eb1ng m\u00e1y s\u1ea5y t\u00f3c. Qu\u00e9t m\u1eadt \u0111\u1ed9 v\u00e0 \u0111\u00e1nh gi\u00e1 \u0111\u1ecbnh l\u01b0\u1ee3ng \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n l\u1ea7n l\u01b0\u1ee3t b\u1eb1ng m\u00e1y qu\u00e9t TLC v\u00e0 ph\u1ea7n m\u1ec1m winCATS phi\u00ean b\u1ea3n 1.4.0. Ph\u01b0\u01a1ng ph\u00e1p s\u1eafc k\u00fd l\u1ecfng \u0111\u1ec3 \u0111\u1ecbnh l\u01b0\u1ee3ng doxycycline \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng HPLC (Shimadzu, LC-2030 C 3D, Nh\u1eadt B\u1ea3n).<\/p>\n\n\n\n

    T\u00c0I LI\u1ec6U THAM KH\u1ea2O<\/strong><\/p>\n\n\n\n

    1. Zi W, Xuejun C. Preparation of core\u2013shell molecular imprinting polymer for lincomycin A and its application in chromatographic column. Process Biochemistry.<\/em> 2015; 50(7) 1136-1145. DOI10.1016\/j.procbio.2015.04.013.<\/li>
    2. B\u1ed9 Y t\u1ebf. Chuy\u00ean lu\u1eadn vi\u00ean n\u00e9n methyl prednisolon. D\u01b0\u1ee3c \u0111i\u1ec3n Vi\u1ec7t Nam V t\u1eadp 1. <\/em>T\u00e1i b\u1ea3n l\u1ea7n th\u1ee9 5. B\u1ed9 Y t\u1ebf; 2017: 635-636.<\/li>
    3. Serni, E. Pistelli, L.  Bertoli, A. Comparison between thin-layer chromatography and overpressured layer chromatography fingerprints of commercial essential oils and accelerated solvent extraction plant extracts. Akademiai Kiado ZRt. <\/em>2021;  34(2), 113-120.<\/li>
    4. Lensi, K. Thomas, L. Ariaya, H. Ayenew, A. High performance thin layer chromatography (HPTLC) method development and validation for determination of doxycycline hyclate in capsule and tablet formulations. Acta Chromatographica<\/em>. 2022; 34(3), 287\u2013295. Online ISSN: 2083-5736.<\/li><\/ol>\n\n\n\n

      BM. H\u00d3A PH\u00c2N T\u00cdCH – KI\u1ec2M NGHI\u1ec6M<\/p>\n","protected":false},"excerpt":{"rendered":"

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